Heavy/Light-Chain Analysis of Monoclonal Gammopathies

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Heavy/Light-chain analysis of monoclonal gammopathies.

Monoclonal gammopathies (M proteins) are associated with an extraordinarily broad array of clinical conditions that range from monoclonal gammopathy of undetermined significance (MGUS) to multiple myeloma. Three basic goals of the methods used to investigate M proteins are detection, immunochemical characterization, and quantification. Serum protein electrophoresis (SPE) techniques have undergo...

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Monoclonal gammopathies

Clinical background The finding of a monoclonal immunoglobulin in the serum defines the so-called monoclonal gammopathy. Monoclonal components are homogeneus immunoglobulins whole (intact) or fragmented, produced by a single expanded plasma cell clone. MCs present unique physicochemical homogeneity, which is reflected in their immunological homogeinity. Immunological homogeinity by electrophore...

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The monoclonal gammopathies.

Each monoclonal protein (M-protein, or myeloma protein) consists of two heavy polypeptide chains of the same class and subclass and two light polypeptide chains of the same type. Electrophoresis on cellulose acetate membranes is satisfactory for screening, although agarose electrophoresis is more sensitive for detecting small M-proteins. Immunoelectrophoresis should be performed when myeloma, m...

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Monoclonal gammopathies of undetermined significance.

Monoclonal gammopathy of undetermined significance (MGUS) is characterized by the presence of a monoclonal protein (M-protein) without evidence of multiple myeloma (MM), Waldenstrom's macroglobulinemia (WM), amyloidosis (AL), or a related plasma cell proliferative disorder. Agarose gel electrophoresis followed by immunofixation is recommended for recognition of an M-protein. Monoclonal gammopat...

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Ankylosing spondylitis and monoclonal gammopathies.

From 1960 to 1990, 557 patients with ankylosing spondylitis (428 men, 129 women) were diagnosed and indexed in the department of rheumatology. Monoclonal gammopathies were found in seven (five men, two women) patients (1.3%). With one exception, ankylosing spondylitis preceded monoclonal gammopathies by many years. The distribution of the isotypes of the mIg found in these seven patients was st...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 2009

ISSN: 0009-9147,1530-8561

DOI: 10.1373/clinchem.2009.132753